Molecular Typing of Dermatophytes Isolated from Pupils and Staff Members of Some Selected Schools in Anambra State, Nigeria

Dermatophytes are the fungal pathogens of human and animals infecting keratinized tissues body namely skin, hair nails. They include species Trichophyton, Microsporum Epidermophyton. This study was aimed at molecular typing dermatophytes isolated from school pupils staff members some selected Schools in Anambra East Ayamelum L.G.As State Nigeria. One thousand (1000) samples (scalp/hair, nail, feet, glabrous skin groin/perianal) were collected both gender age bracket (1 to 10, 11 20, 21 30 etc ) years that showed visible signs infection located these two Local Government Areas. Standard procedures employed processing test inoculation on dermatophyte medium. The plates incubated room temperature (25 – 270C) for 7 10days observation growth. Colonial, morphology, studies sequencing used identification. Sensitivity performed using sterilized discs (6mm) prepared whatman No. 1 filter paper, impregnanted with different concentrations (25mg, 50mg, 100mg 200mg) terbinafine, itraconazole, ketoconazole, fluconazole griseofulvin dissolved 2% dimethylsuphuroxide (DMSO). Molecular as confirmatory tests dermatophytic isolates Sanger method. results show includes: Trichophyton tonsurans, audounii, rubrum, canis, violaceum, verrucosum, mentagrophytes, Epidermorphyton flocossum. Results also revealed nucleotide sequences genetic relationships between schools. PCR-RFLP gold standard diagnosis Confirmation Source can aid initiating prompt appropriate antifungal therapy. Phylogenetic tree drawn isolates.